Collection of a few laboratory techniques

This Course on Genetic Engineering covers the basic concepts of cloning. The tools used for cloning and the methods for the process is explained. In addition, basic techniques in Genetic Engineering like PCR, Sequencing of DNA & proteins, Blotting techniques are discussed

Expected outcomes:   

After completing the course, the learners will be able to 

  • explain the concept of cloning
  • describe the role of enzymes used in cloning
  • distinguish between genomic DNA and cDNA libraries
  • summarize the properties of common cloning vectors
  • illustrate the processes of PCR, sequencing of DNA & proteins
  • compare the various blotting techniques used for transferring nucleic acids & proteins

The outline of this course is aligned with the University of Mumbai T.Y.B.Sc. Zoology Semester VI-Course 17 USZO603 Paper 3 Unit 2 - Genetic Engineering

UoM syllabus for this unit is in the course image


Learning Outcomes:

After completion of this course, the learners will be able to 

  • understand and explain the types of point mutation
  • enlist the various mutagenic agents and illustrate their mode of action
  • describe the various DNA repair mechanisms 
  • explain the regulatory protein domains in eukaryotes

 This course is aligned with the University of Mumbai T.Y. B.Sc. Zoology Semester VI - Course 17 USZO603 Paper 3 Unit 1 - Molecular Biology.

UoM syllabus for this Unit